Telephone: +44 (0)131 242-9111
Professor Lee Smith’s group focus upon all aspects of male reproductive health, specialising in identifying genetic and hormonal control signals that support male fertility and steroid hormone (testosterone) production
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Latest News from the Smith Group!
March 2014: Our recent paper on the role of Azi1 in cilia and sperm flagella formation receives a Faculty 0f 1000 ‘article recommendation‘
March 2014: Four further papers In Press so far this year (see publications below).
January 2014: First 2014 paper is published!
The adult testis is essentially a factory, which produces two key products, – the synthesis and secretion of steroid hormones, notably testosterone (made by testicular Leydig cells), and the production of mature sperm from spermatogonial stem cells (a process known as spermatogenesis). My group is working both to understand how these processes are regulated, and to develop new mechanisms to support both male fertility and life-long health and wellbeing.
Development of functional sperm from stem cells within the testis is a complex process. The genes that control this in men remain largely uncharacterised because it is very difficult to identify them. Our group use mouse models of male infertility to identify genes essential for sperm production. Through this process we are developing an understanding of the genetic networks and processes underlying testis function; knowledge that will be of benefit both to development of future treatments for male infertility and development of non-hormonal male contraceptives.
Steroidogenesis – testosterone production
In the male, androgens (such as testosterone) play significant roles both in male development and in adult reproductive function and general health. A reduction in androgen action during key stages of development results in incomplete physical masculinisation which can have life-long impacts (fetal programming), whilst disruption in androgen action in adulthood can lead to infertility, and has also been linked to several other widespread chronic conditions such as cardiovascular disease, obesity, depression and age-related deterioration in health.
It has been known for many years that testosterone signalling plays an essential role in regulating the process of spermatogenesis. However how this signal impacts upon each testicular cell-type has remained elusive. Testosterone acts by binding to the androgen receptor (AR) a single copy gene located on the X-chromosome which is expressed in several cell-types of the testis, but not germ cells (sperm). Thus testosterone’s effect upon sperm development must be indirect, acting via the supporting cell-types.
We have used the Cre/lox Recombination system to generate mouse models with selective ablation of AR from every somatic (supporting) cell-type of the testis. Our extensive analyses have characterized a previously undescribed paracrine-signalling network within the testis, which acts to support both sperm production and testosterone production.
Our current work is focused upon dissecting the mechanisms underlying this, using novel transgenic mouse models coupled to a wide variety of cutting edge molecular genetic and endocrinological techniques. In addition to this, we have extended our studies to investigate mouse models with targeted deletion of AR in the other body systems, including the cardiovascular system, adipose tissue and prostate.
Together, these investigations highlight a body-wide and lifelong role for androgen-signalling in supporting lifelong health and wellbeing in men. Our future focus is now on understanding how androgen production is controlled within the testis and how this influences not only male fertility, but also other important clinical problems such as cardiovascular disease, diabetes, and age-related conditions.
A Section through an adult testis showing seminiferous tubules (outlined by smooth muscle actin – blue) containing developing sperm (DNA – Green). Note that Androgen (testosterone) Receptor (Red) is expressed in several somatic cell types but is absent from developing sperm. Testosterone control of spermatogenesis therefore relies on a complex network of signals between these supporting cell types and the developing sperm.
Current research projects
- The Genetic and Hormonal Control of Male Reproductive Health and Fertility.
- Testis Development and Function in Relation to Disorders of Reproductive and General Health in Males (with Professor Richard Sharpe).
Selected Recent Publications
Smith LB, and Walker WH. (2014) The Regulation of Spermatogenesis by Androgens. Seminars in Cell and Developmental Biology Mar 2. pii: S1084-9521(14)00024-X. doi: 10.1016/j.semcdb.2014.02.012.
Sauter K, Pridans C, Sehgal A, Tsai YT, Bradford B, Raza S, Moffat L, Gow D, Beard P, Mabbott N, Smith LB, and Hume D. (2014) Pleiotropic Effects Of Extended Blockade Of Csf1r Signalling In Adult Mice Journal of Leukocyte Biology (In Press)
O’Hara L, York JP, Zhang P, and Smith LB (2014) Targeting of GFP-Cre to the mouse Cyp11a1 locus both drives Cre Recombinase expression in steroidogenic cells and permits generation of Cyp11a1 knock out mice. PLoS ONE 9(1): e84541. doi:10.1371/journal.pone.0084541
Smith LB and Walker WH. (2014) Hormone Signaling in the Testis. In Knobil and Neill’s Physiology of Reproduction (Fourth Edition) (In Press)
Mitchell RT, Camacho-Moll M, Macdonald J, Anderson RA, Kelnar CJH, O’Donnell, M, Sharpe RM, Smith LB, Grigor KM, Wallace WHB, Stoop H, Wolffenbuttel KP, Donat R, *Saunders PTK and *Looijenga LHJ. (2014) Intratubular germ cell neoplasia of the human testis: heterogeneous protein expression and relation to invasive potential Modern Pathology doi: 10.1038/modpathol.2013.246
Hall EA, Keighren M, Ford MJ, Davey T, Jarman AP, Smith LB, Jackson IJ and Mill P. (2013) Acute versus chronic loss of mammalian Azi1/Cep131 results in distinct ciliary phenotypes. PLoS Genetics PLoS Genet 9(12): e1003928. doi:10.1371/journal.pgen.1003928 (FACULTY 1000 Citation)
Smith LB, Mitchell RT and McEwan IJ. (2013) Testosterone: From Basic Research to Clinical Applications. Springer Briefs – Reproductive Biology Series
Mitchell R, Sharpe RM, Anderson RA, McKinnell C, Macpherson S, Smith LB, Wallace WHB, Kelner C, and van den Driesche S (2013) Diethylstilboestrol exposure does not reduce testosterone production in human fetal testis xenografts. PLoS ONE 8(4): e61726. doi:10.1371/journal.pone.0061726
Smith LB, Milne L, Nelson N, Eddie S, Brown P, Atanassova N, O’Bryan MK, O’Donnell L, Rhodes D, Wells S, Napper D, Nolan P, Lalanne Z, Cheeseman M and Peters J. (2012) KATNAL1 Regulation of Sertoli Cell Microtubule Dynamics is Essential for Spermiogenesis and Male Fertility. PLoS Genetics PLoS Genet 8(5): e1002697. doi:10.1371/journal.pgen.1002697
O’Donnell L, Rhodes D, O’Connor AE, Smith SJ, Clark B, Borg C, Whittle B, Merriner DJ, Smith LB, McNally F, de Kretser DM, Goodnow CC, Ormandy CJ, Jamsai D and O’Bryan MK. (2012) An essential role for p80 katanin and microtubule severing in male gamete production. PLoS Genetics PLoS Genet 8(5): e1002698. doi:10.1371/journal.pgen.1002698
Van den Driesche S, Walker M, McKinnell C, Scott HM, Eddie SL, Mitchell RT, Seckl JR, Drake AJ, Smith LB, Anderson RA and Sharpe RM (2012) Proposed Role for COUP-TFII in Regulating Fetal Leydig Cell Steroidogenesis, Perturbation of which leads to Masculinization Disorders in Rodents. PLoS ONE 7(5): e37064. doi:10.1371/journal.pone.0037064
McInnes KJ, Smith LB, Hunger NI, Saunders PTK, Andrew R and Walker BR. (2012) Deletion of the Androgen Receptor in adipose tissue in male mice elevates retinol binding protein 4 and reveals independent effects on visceral fat mass and on glucose homeostasis. Diabetes 2012 Mar 13. [Epub ahead of print]
O’Hara L and Smith LB. (2012) Androgen Receptor Signalling in Vascular Endothelial Cells is Dispensable for Spermatogenesis and Male Fertility. BMC Research Notes 5:16
Dean A, Smith LB, Macpherson S and Sharpe RM. (2012) The effect of dihydrotestosterone exposure during or prior to the masculinization programming window on reproductive development in male and female rats. International journal of Andrology doi: 10.1111/j.1365-2605.2011.01236.x.
Welsh M, Moffat L, Belling K, Renato de França L, Segatelli T, Saunders PTK, Sharpe RM, and Smith LB (2012) Androgen Receptor Signalling in Peritubular Myoid Cells is Essential for Normal Differentiation and Function of Adult Leydig Cells. International Journal of Andrology 35: 25-40
Denison FC, Smith LB, Muckett PJ, O’Hara L,Carling D, Woods A. (2011) LKB1 is an Essential Regulator of Spermatozoa Release During Spermiation in the Mammalian Testis. PLoS ONE 6(12): e28306. doi:10.1371/journal.pone.0028306
Welsh M, Moffat L, McNeilly A, Brownstein D, Saunders PTK, Sharpe RM, Smith LB. (2011) Smooth muscle cell-specific knockout of androgen receptor: a new model for prostatic disease. Endocrinology. 152(9):3541-51.
Smith LB and Saunders PTK (2011) The Skeleton: the new controller of male fertility? Cell. 144(5) 642-643
O’Hara L, Welsh M, Saunders PTK and Smith LB (2011) Androgen Receptor Expression In The Caput Epididymal Epithelium Is Essential For Development Of The Initial Segment And Epididymal Spermatozoa Transit. Endocrinology 152 (2): 718-729 (Faculty of 1000 citation)
- O’Hara L, Welsh M, Saunders PTK and Smith LB (2011) Androgen Receptor Expression In The Caput Epididymal Epithelium Is Essential For Development Of The Initial Segment And Epididymal Spermatozoa Transit. Journal of Clinical Endocrinology and Metabolism – Translational Highlights 96(2) 555
- O’Hara L, Welsh M, Saunders PTK and Smith LB (2011) Androgen Receptor Expression In The Caput Epididymal Epithelium Is Essential For Development Of The Initial Segment And Epididymal Spermatozoa Transit. Endocrine Reviews – Translational Highlights 32(1) 152
Smith LB (2011) Good Planning and Serendipity: Exploiting the Cre/Lox system in the Testis. Reproduction. 141(2):151-61
Orr B, Vanpoucke G, Grace OC, Smith LB, Anderson RA, Riddick ACP, Franco OE, Hayward SW and Thomson AA. (2011) Expression of Pleiotrophin in the Prostate is Androgen Regulated and it Functions as an Autocrine Regulator of Mesenchyme and Cancer Associated Fibroblasts and as a Paracrine Regulator of Epithelia. Prostate. 71(3):305-17
Smith LB, Hadoke PWF, Dyer E, Denvir M, Brownstein D, Miller E, Nelson N, Wells S, Cheeseman M, and Greenfield A. (2010) Haploinsufficiency of the murine Col3α1 locus causes aortic dissection: a novel model of the vascular type of Ehlers Danlos Syndrome. Cardiovascular Research. doi: 10.1093/cvr/cvq356
Welsh M, Sharpe RM, Moffat L, Atanassova N, Saunders PTK, Kilter S, Bergh A, Smith LB, (2010) Androgen action via testicular arteriole smooth muscle cells is important for Leydig cell function, vasomotion and testicular fluid dynamics. PLoS One 5(10): e13632.
Welsh M, Moffat L, Jack L, McNeilly A, Brownstein D, Saunders PT, Sharpe RM, Smith LB. (2010) Deletion of androgen receptor in the smooth muscle of the seminal vesicles impairs secretory function and alters its responsiveness to exogenous testosterone and estradiol. Endocrinology. 151:3374-85.
Willems A, De Gendt K, Allemeersch J, Smith LB, Welsh M, Swinnen JV and Verhoeven G (2010) Early effects of Sertoli cell-selective androgen receptor ablation on testicular gene expression. Int J. Androl 33(3):507-1
Welsh M, Saunders PT, Atanassova N, Sharpe RM, Smith LB. (2009) Androgen action via testicular peritubular myoid cells is essential for male fertility. FASEB J. Dec; 23(12):4218-30 (Faculty of 1000 citation)
Smith LB, Saunders PTK (2009) What makes a man? The effect of androgen receptors on male fertility. The Biochemist 31:12-15
Welsh M, MacLeod DJ, Walker M, Smith LB and Sharpe RM. (2009) Critical androgen-sensitive periods of rat penis and clitoris development. International Journal of Andrology 32, 1–8
Welsh M, Sharpe RM, Walker M, Smith LB, Saunders PT. (2009). New insights into the role of androgens in Wolffian duct stabilization in male and female rodents. Endocrinology. 150: 2472-2480
Pastorelli LM, Wells S, Fray M, Smith A, Hough T, Harfe BD, McManus MT, Smith LB, Woolf AS, Cheeseman M, Greenfield A. (2009) Genetic analyses reveal a requirement for Dicer1 in the mouse urogenital tract. Mamm Genome. Volume: 20 Issue: 3 Pages: 140-151
Welsh M, Saunders PT, Fisken M, Scott HM, Hutchison GR, Smith LB, and Sharpe RM (2008) Identification in rats of a programming window for reproductive tract masculinization, disruption of which leads to hypospadias and cryptorchidism. J Clin Invest 118: 1479-90
Smith LB, Willan J, Warr N, Brook FA, Cheeseman M, Sharpe R, Siggers P, Greenfield A. (2008). The Maestro (Mro) gene is dispensable for normal sexual development and fertility in mice. PLoS ONE. 3(12):e4091